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SRX366168: GSM1246714: Smad3 ChIP-seq, H441/siTTF-1 cells; Homo sapiens; ChIP-Seq
1 ILLUMINA (Illumina Genome Analyzer IIx) run: 32.7M spots, 1.2G bases, 639.1Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Smad3, Smad4 and TTF-1/NKX2-1 binding sites in H441/A549 lung cancer cell lines
show Abstracthide Abstract
We evaluated the role of TTF-1/NKX2-1 on Smad3 and Smad4 binding in lung cancer cell lines. Overall design: Smad3 binding sites in A549 cells and Smad3, Smad4, and TTF-1/NKX2-1 binding sites in H441 cells were determined by ChIP-seq.
Sample: Smad3 ChIP-seq, H441/siTTF-1 cells
SAMN02378774 • SRS492409 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina Genome Analyzer IIx
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: ChIP were performed as described (Koinuma et al, Mol Cell Biol 29: 172-186, 2009). SMAD3, SMAD4, or TTF-1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.
Experiment attributes:
GEO Accession: GSM1246714
Links:
External link:
Runs: 1 run, 32.7M spots, 1.2G bases, 639.1Mb
Run# of Spots# of BasesSizePublished
SRR101399532,652,7821.2G639.1Mb2015-07-22

ID:
523267

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